FAM199X Knockout cell line (Hep G2)

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FAM199X Knockout cell line (Hep G2) Order

FAM199X Knockout cell line (Hep G2)
Catalog#
YKO-HT7583
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
FAM199X Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
FAM199X Knockout cell line (Hep G2)
Species
Human
Gene Name
FAM199X
Gene ID
139231
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 1 (based on transcript FAM199X-202)
Validation: PCR+Sanger Sequencing
FAM199X Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the FAM199X gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, FAM199X gene knockout FAM199X Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
FAM199X Knockout cell line (Hep G2)
FAM199X Knockout cell line (Hep G2)
Catalog#
YKO-HT7583
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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      inquiry   More details
  • FAM199X Knockout cell line (HCT 116)

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      inquiry   More details

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